I found a site that I thought was both interesting and potentially useful as a reference. It is The Medical Biochemistry Page, hosted by the Indiana University school of medicine. The content of the site is as a sort of reference library; it has links to pages which discuss specific subjects. For example, some of the pages are titled, " Basic Chemistry of Amino Acids", "Thermodynamics Review", "Sphingolipid Metabolism", and "The Cell Cycle", to name a few. I've looked at all of these pages, and their content is comprehensive and understandable.
What is available to the public is a subset of all of the material on the site. They offer a subscription service to access the entire site - rates are published. Whether or not I decide to subscribe to this site remains to be seen, but I have to be honest and say that from what I've seen it might almost be worth the investment.
Thursday, March 1, 2012
Connections to Past Knowledge
I had a question about a specific clinical test last week and its relationship to the material we'd been discussing in class. In the previous lecture we'd learned about multi-subunit proteins with respect to Quarternary structures. In the lecture, multiple types of polymers - dimers, trimers, and tetramers - are said to be common. When I saw the term "dimer", it made me think of a clinical test that is run to rule out pulmonary embolism: a d-dimer. It is actually used in a number of different situations, including deep vein thrombosis (DVT) detection, and in the presence of stroke.
The test itself measures the level of Fibrin degradation. Fibrin is a non-globular protein that is involved in the clotting process - to actually actively work, Fibrinogen is converted to Fibrin by Thrombin, an enzyme involved in the clotting process.
A high Fibrin level usually indicates that further testing be done to determine whether or not a thrombus or embolus is present. Unfortunately, the d-dimer test itself is not always accurate. One of the problems with it is that conditions exist where false levels can be detected for a number of reasons including genetic makeup, presence of statins in the blood, and age to name a few. One condition where true high levels exist, however, is in a situation where the clotting cascade is behaving in such a way that its ability to produce Fibrin (from Fibrinogen, its precursor) is hyperactive.
In addition to function in clotting, Fibrin is also involved in platelet aggregation - its role here is in the signaling process of producing a hemostatic plug.
It was a "light-bulb" moment and I was surprised that I was able to make a connection to this from material being discussed in the classroom.
The test itself measures the level of Fibrin degradation. Fibrin is a non-globular protein that is involved in the clotting process - to actually actively work, Fibrinogen is converted to Fibrin by Thrombin, an enzyme involved in the clotting process.
A high Fibrin level usually indicates that further testing be done to determine whether or not a thrombus or embolus is present. Unfortunately, the d-dimer test itself is not always accurate. One of the problems with it is that conditions exist where false levels can be detected for a number of reasons including genetic makeup, presence of statins in the blood, and age to name a few. One condition where true high levels exist, however, is in a situation where the clotting cascade is behaving in such a way that its ability to produce Fibrin (from Fibrinogen, its precursor) is hyperactive.
In addition to function in clotting, Fibrin is also involved in platelet aggregation - its role here is in the signaling process of producing a hemostatic plug.
It was a "light-bulb" moment and I was surprised that I was able to make a connection to this from material being discussed in the classroom.
PDB Explorer Topic - Anthrax
This blog entry assignment is to go to the PDB Explorer website, find a protein that is of interest, and briefly describe it.
The protein I found for this entry is the Anthrax toxin. Its PDB identifier is 1ACC, and it is produced by gram-negative Bacillus anthracis bacterium. The toxin is stored in spores which is released when the spores are disturbed or agitated in some way. It is made up of three proteins including a Protective Antigen (PA), an Edema Factor (EF), and a Lethal Factor (LF) . The PA is what delivers the toxin to cells that are being targeted. Additionally, it is broken into four domains containing 735 residues. Each of the domains is responsible for a specific characteristic task with respect to the mechanism of delivering the toxin into the targeted cells.
Because of the fairly rapid delivery of toxins due to the architecture of the protein, it is considered a primary threat as it can be used in the production in a biological weapons system.
The protein I found for this entry is the Anthrax toxin. Its PDB identifier is 1ACC, and it is produced by gram-negative Bacillus anthracis bacterium. The toxin is stored in spores which is released when the spores are disturbed or agitated in some way. It is made up of three proteins including a Protective Antigen (PA), an Edema Factor (EF), and a Lethal Factor (LF) . The PA is what delivers the toxin to cells that are being targeted. Additionally, it is broken into four domains containing 735 residues. Each of the domains is responsible for a specific characteristic task with respect to the mechanism of delivering the toxin into the targeted cells.
Because of the fairly rapid delivery of toxins due to the architecture of the protein, it is considered a primary threat as it can be used in the production in a biological weapons system.
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| Anthrax Protecttive Antigen - Biological Assembly Retrieved from http://www.pdb.org under Fair Use guidelines |
References:
Froude, J., Thullier, P., & Thibaut, P. (2011). Antibodies Against Anthrax: Mechanism and Clinical Applications. Toxins, 3, 1433-1452. doi:10.3390/toxins3111433
Helgason, E., Okstad, O.A., Caugant, D., Johansen, H.A., Fouet, A., Mock, M., Hegna, I., & Kolsto, A. (2000). Bacillus antrhacis, Bacillus cereus, and Bacillus thuringiensis - One Species on the Basis of Genetic Evidence. Applied and Environmental Microbiology, 66(6):2627. doi:10.1128/AEM.66.6.2627-2630.2000.
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